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To further simulate different modes of HSV infection, the team devised two variants of this organoid system: a submerged infection model, in which epidermal cells grow in a monolayer submerged in culture media to which HSV is added (this would simulate initial infection of HSV through a break in the skin, for example), and an air-liquid interface (ALI) model, in which the organoids are repositioned to the surface of the culture media, which induces stratification in the air-exposed epidermal tissuein this ALI model, HSV was added to the media side of the tissue (i.e

2000;476:30611 [cited 2020 mar 12] Available from: Yang Z, Wang Z-E, Doulias P-T, Wei W, Ischiropoulos H, Locksley RM, et al

10.1111/j.1365-313X.2008.03761.x

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