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The mevalonate pathway, a metabolic target in cancer therapy

The elongated peptide was cleaved from the solid support using TFA with appropriate scavengers as indicated in each case, precipitated by addition of cold diethyl ether/heptane 1/1 v/v (20 mL per mL of TFA cocktail), and recovered by centrifugation

Seifried HE, McDonald SS, Anderson DE, Greenwald P, Milner JA

We found decreased glycine levels and an increased serine-to-glycine ratio in the senescent cells, consistent with the decreased SHMT2 level in these cells (Fig 6C and D)