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Description

Mice for an immunohistochemistry study were transcardially perfused with phosphate-buffered saline, followed by 10% (v/v) neutral buffered formalin (NBF), with a steady flow rate of 10.0 ml/min using an IPC Digital Peristaltic Pump (Ismatec, Germany)

Nayan Patel: Okay

It accounts for 75% of deaths associated with cutaneous cancers

Fixation was carried out by incubating the cells with 4% paraformaldehyde in PBS containing 0.1% saponin for 45 min at 4 C, followed by washing with PBS supplemented with 0.1% saponin and 1% BSA (SBP)