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The current standard operating procedure by the European Center for the Validation of Alternative Methods (ECVAM) recommends the use of MTT reduction as parameter to make prediction on the skin irritancy of substances using EpiSkin TM test method, while IL-1 endpoint is regarded as a useful adjunct to MTT assay 48

Ahmed and El-Sayed 8 indicated that dietary black seed supplementation successfully improves the body gain percentage, feed intake, and serum biochemical and immunological parameters in rats