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To assess the acceptance of the Vaccine-Associated Feline Sarcoma Task Force of the American Association of Feline Practitioners (AAFP) recommendations that include not injecting vaccines interscapularly (published in 1999) by veterinarians, a study including 392 cats with FISS compared the anatomical locations of tumours between cases with FISS diagnosed before and after the publication of these recommendations (Vaccine-Associated Feline Sarcoma Task Force, 1999)

Sections were then briefly immersed in wash buffer and incubated with a mixture of guinea pig anti-insulin and mouse anti-glucagon (both at 1:600 dilution in antibody diluent from Cell Signaling) and incubated for 90 min at 37 C

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