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Cell isolation was performed at room temperature

Vincent HK, Bourguignon CM, Vincent KR, Weltman AL, Bryant M, Taylor AG

The following are known triggers of mast cell degranulation (in addition to IgG activation via pathogen or allergen):[ref] Acetylcholine Complement fragments (C3, C4, C5) Various drugs, environmental toxins (MRGPRX2 receptor, details below) Peptides (including endorphin, leptin, PTH, and more) Physical conditions (cold, heat, pressure, stress, and vibration)