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2.4 Sample preparation For LCQTOF-MS/MS analysis, 1 mg of powder or homogenized tablet was dissolved or extracted using 5 mL of a 1:1:1 (v/v/v) mixture of water/methanol/acetonitrile, assisted by ultrasonication for 10 min, and afterwards filtered by a Whatman 0.2 m pore size polytetrafluoroethylene (PTFE) filter medium (GE Healthcare, Chicago, IL, United States)

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Additionally, results showed little change in GH or IGF-1 levels, but there was a noted increase in leptin levels on day 15 indicating reduced appetite ( GHRP-2 and Weight Gain An additional study conducted by Thang Long et

Because spontaneous mutation is common, family history of anemia may be absent