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Studies have shown that silencing the anti-chondrogenic factor miR-221 can efficiently promote human BMSC chondrogenesis in vitro and in vivo , even without the need for the chondrogenic inducer TGF- ( Although some gene therapy applications have shown initial success, and the emergence of new gene editing technologies has brought IDD treatment to a new starting point, many obstacles still need to be overcome, such as safety concerns, high cost, and low transfection efficiency

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