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In Phase II detoxification, glutathione S-transferase enzymes conjugate GSH to electrophilic xenobiotics and endogenous metabolites, facilitating their excretion

At 18 weeks post-soman exposure, SAR treatment revealed pronounced antioxidant and anti-inflammatory properties, significantly reducing serum and CSF biomarkers of oxidative stress, such as improving the GSH/GSSG ratio and lowering levels of pro-inflammatory cytokines and chemokines including MCP-1, TNF-, IL-6, IL-1, IL-18, and IL-17A

However, the approach suffers from significant limitations: a time-consuming analytical workflow requiring multiple chromatographic runs per sample, and compromised mechanistic interpretability due to the AUC-based evaluation

Rizzo A, Santoni M, Mollica V, Logullo F, Rosellini M, Marchetti A, et al