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Cytokine Profile Shift: As disease progresses, the adaptive immune response shifts from Th1 dominance to Th2 bias ( In vitro study indicate that -MSH significantly inhibits inflammation and pro-melanocortin POMC production in orbital fibroblasts associated with thyroid-related eye disease ( Hypoxic microenvironment induction effect: Rapidly proliferating fibroblasts and highly metabolically active inflammatory cells within the orbital cavity consume large amounts of oxygen, leading to the formation of localized hypoxic zones ( The core driving role of metabolic reprogramming: The metabolic state of macrophages progressively shifts from aerobic glycolysis during the active phase to a state dominated by oxidative phosphorylation and fatty acid oxidation ( 4.3 Quiescent phase: macrophage states dominated by pro-fibrotic functions lock the fibrosis process During the TED quiescent phase, the microenvironment is dominated by high levels of TGF-, PDGF, and specific extracellular matrix components

Metastasis occurs when tumor cells from the primary site spread and colonize organs throughout the body, resulting in organ dysfunction and failure, as observed in prostate cancer, lung cancer, pancreatic cancer, and other malignancies [2, 3]

This technique applied by our group previously was found to be highly effective in the induction of gastric mucosal injury accompanied by decreased mucosal blood flow, possibly due to decline of microcirculation (51, 52)