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For 3D cultures, Promega recommends CellTiter-Glo 3D Cell Viability Assay, which uses an enhanced lysis buffer specifically formulated to penetrate 3D tumor spheroid structures and release ATP from cells throughout the spheroid

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coli hosts such as XL-1 Blue (Stratagene Cloning Systems, La Jolla, Calif.) cells are transformed with the ligation mixture and spread on culture plates