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PCR amplification was performed in a 50 l reaction as follows: one cycle at 95 C for 10 min, followed by 40 cycles at 95 C for 5 s, 58 C for 25 s, and 72 C for 30 s, with one final extension step at 72 C for 5 min

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Preclinical research in murine senescence models has examined FOXO4-DRI for its capacity to reduce senescent cell burden, with observed improvements in physical function, coat density, and renal histology endpoints in aged subjects