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Western blotting Cells were lysed using RIPA buffer (Thermo Fisher Scientific, Waltham, MA, USA) containing protease inhibitors and phosphatase inhibitors (Beyotime, Nanjing, China), and protein concentrations were measured using a BCA protein assay kit (Thermo Fisher Scientific, Waltham, MA, USA)

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The conclusion is that the trispecific SAR442257 operates via dual targeting of CD38 and CD28 on myeloma cells and of CD3 and CD28 on T lymphocytes, showing superior MM killing compared to bispecific antibodies

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