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After removal of the overlying meninges and blood vessels, spinal cords were cut into small pieces, dissociated with 0.05% collagenase I (Thermo Fisher Scientific, Foster City, CA, USA) for 15 min at 37 C, and then processed to produce epSPC neurospheres as previously described 6,18

Furthermore, higher cortisol levels in preterm newborns with sepsis are associated with circulatory collapse and death, suggesting decreased vascular sensitivity to cortisol