oxiselect total glutathione assay kit (GSSG/GSH) MBS168487 from MyBioSource.com Glutathione Peroxidase (GPX) Activity Assay

10.1167/iovs.07-0037 135

Redundancy in bacterial resistance to ROS is a relatively common phenomenon, including the need to eliminate five individual enzymes in Salmonella enterica Serovar Typhimurium to exhibit a phenotype in the presence of hydrogen peroxide (Hbrard et al., 2009) and simultaneous disruption of four enzymes in Bacillus anthracis to observe a phenotype in the presence of superoxide (Cybulski et al., 2009)

Then, 500 L of the supernatant was removed, 500 L of isopropyl alcohol was added (supernatant:isopropyl alcohol = 1:1), the mixture was allowed to stand for 5 min, and the mixture was centrifuged at 12,000 rpm at 4 for 10 min

Djurkovi-Djakovi O, Bobi B, Nikoli A, Klun I, Dupouy-Camet J (2013) Pork as a source of human parasitic infection