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CRISPR-Cas12a (Clustered Regularly Interspaced Short Palindromic Repeats CRISPR associated protein 12a) biosensors achieved 96.5% sensitivity and 99% specificity for Severe Acute Respiratory Syndrome Coronavirus 2 (SARS-CoV-2) detection in saliva (n = 352 patients) relative to Reverse Transcription quantitative Polymerase Chain Reaction (RT-qPCR), showcasing the diagnostic potential of integrated biosensing approaches ( 2.2 Optical sensors Optical nano-biosensors detect analyte-receptor interactions through light absorption, fluorescence, surface plasmon resonance, and refractive index changes, offering real-time and highly sensitive detection for clinical and PoC applications (Mostufa et al., 2024)

Apr 1983;15(4):337-346

While generation and application of antibodies are restricted to physiological conditions, aptamers can be generated under varying conditions during their in vitro selection

Kalisiska E, Budis H (2019) Manganese, Mn