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Castaldo L, Izzo L, Narvez A, Rodrguez-Carrasco Y, Grosso M, Ritieni A

Premedication protocols help reduce the frequency and severity of many side effects

The eluted proteins were subjected to size-exclusion chromatography (SEC) with a Superose 6 Increase 10/300 GL column (Cytiva) in the final buffer containing 25 mM HEPES pH 7.5, 150 mM NaCl and 1 mM DTT (buffer A, for biochemical assays) or 5 mM DTT (buffer B, for cryo-EM)