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Ancient Chinese medicine, for example, used the plant for musculoskeletal conditions and to relax and harmonize the mind and body [3], while the Greeks and Romans used it for recreational consumption and medical use [4]

In mice model single dosage of 150 mg per kg of cyclophosphamide was administered intraperitoneally then different icariin doses were administered intraperitoneally such as 5 mg, 25 mg, and 50 mg per kg were shown to reduce visceral reflexes and nociception

Then, acetonitrile was added at 85 C until the components were dissolved completely

See Box 1 for the definition of sporadic AD and how it differs from the familial form of AD