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Inhibition of glutaminolysis by Gln depletion, by chemical inhibition of the critical regulators of Gln metabolism such as GLS and MYC, or genetic knockdown of these genes and Gln transporters resulted in the inhibition of the essential CSC-driving signaling mechanisms (e.g., WNT/-catenin

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These findings can be explained by the interaction between membrane anchoring moieties, such as cholesterol and fatty acids present on the surface of LPNs, with the hydrophobic tail regions of phospholipids within the cell membrane 53

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