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Pelleted bacterial cells were lysed in 30 ml of lysis buffer (20 mM Tris-Cl (pH 7.5), 0.1% Triton X-100, 1 mg/ml lysozyme) for 20 min and sonicated, and insoluble molecules were removed

By producing inflammatory mediators that are lethal to tumor cells, such as TNF-Alfa, MIP-1 ALFA, H2O2, and NO, chemokines like CXCL8, CXCL1, CXCL2, and CXCL3 attract it to the tumor microenvironment [55]