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In addition, the day before running the assay, the Seahorse XF Sensor Cartridge was hydrated with sterile water followed by the calibration step (200 L per well of Seahorse XF Calibrant Solution) the day of the experiment in a non-CO 2 37 C incubator

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We highlight how therapeutic manipulation of methionine availability, GCN2 signaling and ROS produces highly context-dependent outcomes across immune checkpoint blockade and adoptive cell therapy settings in solid tumors