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LC-MS/MS separation of endocrine disrupting compounds (EDCs) was performed using a 50 mm 2.1 mm (id), 1.8 m particle (dia) Titan C18 reversed-phase UHPLC column (Supelco, Bellefonte, PA) in combination with a guard column equipped with a guard column insert containing C18 stationary phase complementary with the analytical column

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While in healthy individuals, ROS levels are naturally kept low, unhealthy lifestyle choices, diseases, pollutants, and other stressors can lead to high levels of ROS