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gRNA was transcribed using a MAXIscript T7 kit (Invitrogen, #AM1314)

Salmonella typhimurium translocates flagellin across intestinal epithelia, inducing a proinflammatory response

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The supernatant was collected, and protein was quantified using the Pierce BCA Protein Assay Kit (Thermo Scientific, 23227) before storing at 80 C