l carnitine and tma Revisiting the Role of in Heart Disease Through the Lens of the Gut Microbiota Trimethylamine N-Oxide as a Potential

These buffers offer the opportunity to optimize and fine tune weak and transient interactions between short amino acid sequences in different proteins in terms of stringency

[DOI] [PubMed] [Google Scholar] 14.Ames BN, Cathcart R, Schwiers E, Hochstein P

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For each sample, 20 g protein extract in SDS-buffer was denaturated at 60 C for 5 min, followed by reduction with 10 mM (final concnetration) dithiothreitol (DTT) at 37 C under constant shaking (700 rpm) for 30 min and cysteine alkylation with 50 mM chloroacetamide (final concentration) in the darkness for 30 min