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Subsequently, the buffer of the eluted fractions was exchanged using a Vivaspin 20 Ultra centrifugal unit (30 kDa cut-off, Merck Life Science) and the proteins were further purified using a gel-filtration column (SuperdexTM 200 HR 10/300 column, GE Healthcare Life Sciences) in 20 mM Tris-HCl pH 7.4, 300 mM NaCl, 5% glycerol, and 1 mM dithiothreitol (DTT)

AMP-activated protein kinase activates p38 mitogen-activated protein kinase by increasing recruitment of p38 MAPK to TAB1 in the ischemic heart

Animal studies at effective doses showed no overt toxicity, but this cannot be extrapolated to human safety

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