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Description

Watanabe K, Tachibana O, Sata K, Yonekawa Y, Kleihues P, Ohgaki H

Third, LCA-induced biofilm formation occurred in a medium that is effective for culturing a broad range of gut bacteria in vitro 26 , suggesting that it captures major features of the nutritional profile of typical human colon contents

This includes pro-inflammatory cytokines ( IL-1 and TNF- ), chemokines ( CCL2 ), fibrogenic mediators ( TGF- and connective tissue growth factor), and antioxidant enzymes ( SODs , GPX , TrxR1 , NQO1 , and HO-1 ) [35]

It is important to test the density profile that is formed from such discontinuous gradients, but once satisfactory conditions are established the profile will be highly reproducible