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Glucose-oxidase like catalytic mechanism of noble metal nanozymes

After incubation, the beads were washed with 1 ml of ice-cold phosphate-buffered saline (PBS), pH 7.4, three times, and the beads were eluted in sample loading buffer by boiling for 10 min at 100 C

The natural FX a polypeptide used for the comparison was the natural recombinant FX a generated by the cloning of the FX gene defined in SEQ ID NO: 1 and expressed in CHO cells as a polypeptide with the secu Amino acids defined as amino acids 1 - 139 and 195 - 448 of SEQ ID NO: 134, as described in E jemp lo s 1 -3 (ie, the FXWT polypeptide)

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