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This dialysis bag was then immersed in either 200 mL of phosphate-buffered saline (PBS) or a 200 mL aqueous solution of hydrogen peroxide (H 2 O 2 ) at a concentration of 10 mM, all while being maintained in a shaking culture incubator at 37 C

Interestingly, embryonic arrest was observed only when FAT-2 was depleted ubiquitously in wild type worms (Supplementary Figure S4C)

L-carnitine supplementation L-Carnitine as a sports supplement is used for its role in transport of fatty acids into the mitochondria , with the aim of optimizing energy metabolism and improving recovery