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These early changes are subtle and easily attributed to placebo effect

based on consistent formation of -helical structure in initial rounds of structure calculations in which no hydrogen bond restraints were defined), inter-molecular PRE restraints (4 non-interacting copies of the spin label to account for different spin label orientations), inter- and intra-molecular NOE restraints, and ambiguous distance restraints for residues showing CSPs upon binding

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In the future, it will be necessary to examine how Sirt1 regulates the Shh/Gli-1 signaling pathway and to confirm the results of the present study in vivo