glutathione affinity chromatography protocol GST-Tagged Protein Purification: Comprehensive Guide & Uses Scheme for the standard protocol

19 (Pt B): 3347

E11O0028 Product Name Bovine Oxidized Glutathione ELISA kit Species Bovine Product Size 48 Tests / 96 Tests Concentration 250-5000 pg/mL Sensitivity 1.0 pg/mL Principal Competitive ELISA Sample Volume 100 ul Sample Type Serum, plasma, cell culture supernatants, body fluid and tissue homogenate Assay Time 90 minutes Platform Microplate Reader Conjugate HRP Detection Method Colorimetric Storage 2-8C Kit Components MATERIALS SPECIFICATION QUANTITY MICROTITER PLATE 96 wells stripwell ENZYME CONJUGATE 6.0 mL 1 vial STANDARD A (0.5mL) 0 pg/mL STANDARD B (0.5mL) 250 pg/mL STANDARD C (0.5mL) 500 pg/mL STANDARD D (0.5mL) 1000 pg/mL STANDARD E (0.5mL) 2500 pg/mL STANDARD F (0.5mL) 5000 pg/mL SUBSTRATE A 6 mL SUBSTRATE B STOP SOLUTION WASH SOLUTION (100 x) 10 mL BALANCE SOLUTION 3 mL Principle of the Assay GSSG ELISA kit uses an anti-GSSG antibody and an GSSG-HRP conjugate in a competitive enzyme immunoassay method

Ublinskiy, A

The Z-factor is a dimensionless, simple statistical characteristic for each HTS assay