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The samples were subsequently centrifuged at 4 C and 2000g for 10 min, after which the supernatant was collected for subsequent measurements

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As with the female side, the evidence that antioxidant supplements change outcomes is low to very low certainty rather than settled: the 2022 Cochrane review of antioxidants for male subfertility rated its findings as low-to-very-low certainty evidence

GSH also protects cells by supporting DNA repair, possibly by stabilizing repair enzymes such as DNA polymerase (Rooseboom and Schaaf, 2002)