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2.3 Sample collection, preparation, and processing for proteomics analysis The sample collection, storage, assessment of semen parameters, and protein extraction were performed following the applicable guidelines and regulations of the WHO laboratory manual (2010)

Schistosoma , P28 glutathione-S-transferase (P28GST), combines the activities of antioxidant glutathione S-transferase and prostaglandin D synthase [115,116]

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The identified proteins are then statistically analyzed for statistical significance and used in pattern recognition algorithms to identify a pattern of proteins associated with the disease or drug effect