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2 Materials and methods 2.1 The experimental animals Currently, we included 36 adult male albino Wistar rats (with a body mass range equal to 190230 g, 810 weeks of age) supplied by the Faculty of Veterinary Medicine, Zagazig University. Rats were kept in hygienic laboratory conditions, including temperature (22C 3C), relative humidity (60% 10%), and normal light/dark cycle

Tian CJ, Kim SW, Kim YJ, et al

Type IV pili, conjugation genes, and plasmid-borne type III effectors were induced early in both niches, suggesting their niche-independent role in initial establishment