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In situ hybridization (smFISH) using RNAscope probes In situ hybridizations were performed using RNAscope Probe-Mm- Hgf -No-XHs (target region: 6-2185 nucleotides, NM_001289458.1), Probe-Mm- Hgf -C3 (target region: 1203-2113 nucleotides, NM_001289458.1, that is equal to 1120-2030 nucleotides of NM_010427.4) (Table 1)