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8.5.2 In vitro modification In vitro modification is performed directly on purified EVs, offering advantages such as operational controllability and high yield

IT DFO could potentially cause neurotoxicity if the local concentration is too highhigh doses of systemic DFO have been associated with neurotoxic effects (e.g., hearing loss, seizures) (Zhang et al., 2025), and these risks would potentially be higher through direct CNS exposure

Tissue healing generally moves through three stages: inflammation, proliferation, and remodeling