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Our results showed that Li-loaded ENSs gradually released Li within 11 d, at concentrations ranging from 0.02 to (3.64 0.10) mmol/L, and upregulated the expression of Wnt/ -catenin target genes ( cyclinD1 and c-Myc ) as well as those of Schwann cell markers (growth-associated protein 43 (GAP43), S100 calcium binding protein B (S100B), glial fibrillary acidic protein (GFAP), and SRY-box transcription factor 10 (SOX10)) in differentiated hADMSCs

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A total of 5000 cells in 200 l complete medium was added to each well of a 96-well plate and incubated at 37 C in 5% CO 2 to a maximum confluence of 60% after which the culture medium was replaced by SDS dilutions in three replicates of each concentration