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The detection and quantification of urinary mycotoxin metabolites was performed using a liquid chromatography tandem mass spectrometry (LC-MS/MS) system including binary pumps, auto injector, separation column, and MS/ MS detector LC-MS/MS analyses were performed on a triple quadrupole 8060 (Shimadzu, Kyoto, Japan)

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Absorbance of aqueous layer at 390 nm was measured with blank (5 mL of papain) ( For methionine estimation, 500 mg of sample was autoclaved with 6 mL of 2 N HCl for 1 h