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doi: 10.1172/JCI131931 20 SookoianSPirolaCJ

Clinical trials showed a favorable safety and tolerability profile with no serious adverse events

Surv Ophthalmol 2008, 53 Suppl1:S3-10

Quantitative real-time PCR (qRT-PCR) analysis of neuroprotective gene expression RNA isolation and quality control The manufacturers protocol was followed to extract total RNA from brain tissue ( n = 6 rodents per group) using Sepasol-RNA I Super G reagent (Cat: 09379-84, Nacalai Tesque, Japan)