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Moreover, emerging studies highlight sex-specific epigenetic mechanisms in AD, revealing gender-specific correlations with the disease for CpG sites in genes like TMEM39A and TNXB (169)

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Western blot Cells and tissues were homogenized in ice-cold RIPA (50 mM Tris pH 8.0, 1% nonidet P40 0.5% deoxycholate, 0.1% SDS, 150 mM NaCl or ThermoFisher, 89900) or urea containing phosphatase inhibitor cocktail 3 (Sigma-Aldrich) and protease inhibitor (Roche Diagnostics or ThermoFisher, 78440) and 15 mM NaVanadate in selected experiments