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These approaches ensure a 1 nM effective concentration of the fluorescently labeled nanobody (NbLumSyt1-pH/Nb-At647N or NbLumSyt1-HALO/JF549) on the imaging plate, allowing partial labeling of the Syt1 nanobodies without over-saturating the fluorescent signal enabling single molecule detection

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Indeed, recent studies showed that, in addition to mediating ADCC in in vitro and ex vivo models, isatuximab and daratumumab directly activated NK cells and increased their lytic activity against tumor cells through CD16 and CD38 crosslinking [32, 33]