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Known off-target tendencies: Internal LXXFQG and (V/L)XLFQG-like motifs in your target sequence Slow but real cleavage at LEVLFQA and LEVLFQS significant at overnight scale In GST-PreScission preparations, residual contaminating proteases from production can confound diagnosis Thrombin Consensus: LVPRGS (commonly written as the synthetic LVPRGS)

3D spheroids were fixed with 4% paraformaldehyde/PBS for 45 min at 4 C, washed once with 0.1% Tween-20 in PBS, and suspended in wash buffer (SWB: 2% BSA, 0.1% Triton X-100, 0.025% SDS, 1 PBS)

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Med Phys 34(6):2371, 2007