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Previous work has demonstrated that GFP positive cells in Brn3c:mGFP fish co-label with phalloidin staining and thus can be used a reliable marker of mature hair cells (Uribe et al., 2013)

It is important to note that soreness around incisions is expected to last for several weeks to months and numbness and tingling may disappear rapidly or slowly, often in relation to pre-operative severity of symptoms

If an administrator is concerned with contamination, they may take precautions such as turning off tap water in the bathroom or adding a dye to the toilet water

Cost and Convenience NAC is generally less expensive to produce and is very stable on the shelf