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NAC exerts, proportional to its concentration, a dual role simultaneously increasing both proliferation and apoptosis in HepG2 and 3B cells and interestingly, the levels of protein-bound GSH are decreased by NAC [1]

To confirm that -Glu-Ser-Gly is synthesized directly from glutathione, we further generated GCS knockdown 293T cells deficient in glutathione biosynthesis using RNA interference (Fig

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