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Tail-flick mice assay Mice For analgesic doseresponse experiments, male CD1 mice (2032 g), 68 weeks were obtained from Charles River Laboratories and male C57BL/6 mice (2230 g), 815 weeks were obtained from Jackson Lab (Bar Harbor, ME) and housed 5 mice per cage in a vivarium following an IACUC-approved protocol

Sources Institute of Medicine, Food and Nutrition Board, National Academies of Science, Dietary Reference Intakes (DRI): Recommended Dietary Allowances and Adequate Intakes , USA 2018, Mustafa Vakur Bor, Kristina M von Castel-Roberts, Gail PA Kauwell, Sally P Stabler, Robert H Allen, David R Maneval, Lynn B Bailey Ebba Nexo Daily intake of 4 to 7 g dietary vitamin B-12 is associated with steady concentrations of vitamin B-12related biomarkers in a healthy young population Am J Clin Nutr 2010 91: 3 571-577