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Description

Western blotting Pancreatic cancer cells were lysed and the total protein was extracted with RIPA buffer (Beyotime, Guangzhou, China)

If the vitamin B12 levels normalize, oral replacement may continue

The simplicity of the technique and the highly reproducible nature of the gradients make this a very attractive method

Furthermore, while vascular normalization has emerged as a promising strategy, biomarkers to predict the response to normalization therapies are still not well-established (100)