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[DOI] [PMC free article] [PubMed] [Google Scholar] 247.Mu Y, Li L, Hu SQ

However, a course of injections particularly the course of 12, structured as once weekly for six weeks followed by monthly maintenance builds and maintains consistently higher B12 levels over time

Recombinant human procathepsin B (R&D systems) was activated to mature cathepsin B by incubation at 37 C for 20 min in activation buffer (20 mM sodium acetate pH 5.5, 1 mM EDTA, 5 mM DTT and 100 mM NaCl)

With prudent sunlight exposure and adequate vitamin D supplementation, complemented by diet, you should be on your way to optimal levels of vitamin D