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Description

The antibodies for GGT (1:1000, ab109427, rabbit mAb), GPX4 (1:1000, ab125066, rabbit mAb) and -actin (1:1000, ab8226, mouse mAb), goat anti-rabbit IgG H&L (HRP) (1:5000, ab6721), and goat anti-mouse IgG H&L (HRP) (1:5000, ab205719) were used and obtained from Abcam

This method is an indirect detection method of free radicals, including ROS, in which N,N-Diethyl-para-phenylenediamine reacting with hydrogen peroxide in the serum is measured by the change in the colour as the absorbance at 505 nm, using the Free Radical Elective Evaluator (WISMERLL Co