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These cell lines were maintained in Dulbeccos Modified Eagles Medium (DMEM) (Thermo Scientific, #12100061)low glucose for HepG2 (Thermo Scientific, #31600091), supplemented with 1 % Penicillin/Streptomycin (P/S) and 10 % Fetal bovine serum (FBS) (Natocor)

Am J Clin Nutr 92(6):13691377 Herculano-Houzel S (2011) Scaling of brain metabolism with a fixed energy budget per neuron: implications for neuronal activity, plasticity and evolution

low sodium appears to be more common than high sodium in advancing CKD

The p53(3KR) mutant, lacking acetylation due to lysine-to-arginine substitutions at three residues, decreases expression of SLC7A11 without affecting other p53 targets such as CDKN1A/p21 (involved in cell cycle progression) or BAX (involved in apoptosis)