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The potential of hydrogen for improving mental disorders

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Drucker, D.J

Experimentize to confirm that candidate MIM gets into cell, whether be positioned in the cell, and measure level and the form that has the candidate MIM in the cell with definite candidate MIM.For example, carry out these experiments like what describe in detail among the embodiment 5.For example, in order to measure level and the form of the candidate MIM that is present in the mitochondrion, prepare and analyze the mitochondrial preparation of enrichment of the cell of the candidate MIM processing of using by oneself.Thereby can confirm that the level that is present in the candidate MIM in the line material body increases with time and the dependent mode of dosage after adding external source candidate MIM.In addition, come the variation of the protein level of the mitochondrial sample of self enrichment through using the 2-D gel electrophoresis analysis, and utilize mass spectral characteristi to carry out identification of proteins, as described in the preceding text for the total cell protein quality sample.To be accredited as MIM through the candidate MIM that find to get into cell and be present in the level that raises in the mitochondrion for example.Can make in time course in the checked cell or for example especially the level of the candidate MIM in the mitochondrion with as be related through the mRNA of for example specified protein and other observed cellular change that adjusting proved of protein level