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At that time, the cells were pelleted (400 g , 20 min) and resuspended in a staining solution containing 50 g/mL PI (81845, Sigma-Aldrich Chemie GmbH, Munich, Germany) and 50 U/mL RNase A (EURx, Gdansk, Poland) in PBS

However, phase I reactions are not always sufficient to make the toxin water-soluble enough and in many cases, they render the products more reactive, which makes the toxins potentially more destructive

Although exogenous factors, including infection and inhaled pollutants, could directly oxidize GSH, endogenous disease-specific mechanisms could also prime the process

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